LRRC17 Polyclonal antibody

• Featured Product
• KD/KO Validated

LRRC17 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20918-1-AP

Synonyms

LRRC17, P37NB

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Validation Data Gallery

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  WB analysis of HeLa using 20918-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20918-1-AP (LRRC17 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 20918-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20918-1-AP (LRRC17 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

The immunogen of 20918-1-AP is LRRC17 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRRC17 fusion protein Ag15117
• Full Name: leucine rich repeat containing 17
• Calculated Molecular Weight: 441 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC027903
• Gene Symbol: LRRC17
• Gene ID (NCBI): 10234
• RRID: AB_2878763
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Leucine-rich repeat-containing protein 17 (LRRC17) is an LRR protein predominantly expressed by osteoblasts. LRRC17 acts as a negative regulator of receptor activator of NF-kappaB ligand (RANKL)-induced osteoclast differentiation (PMID: 19336404). It has been reported that low plasma level of LRRC17 is an independent risk factor for osteoporotic fractures (PMID: 27355564).

Publications

Species	Application	Title
mouse	WB	Redox Biol; LRRc17 controls BMSC senescence via mitophagy and inhibits the therapeutic effect of BMSCs on ovariectomy-induced bone loss.; Authors - Fei Liu
human	WB	Exp Ther Med; LRRC17 regulates the bone metabolism of human bone marrow mesenchymal stem cells from patients with idiopathic necrosis of femoral head through Wnt signaling pathways: A preliminary report.; Authors - Da Song ; KD Validated