LPIN1 Monoclonal antibody, PBS Only

LPIN1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G11B10

Cat no : 68124-1-PBS

Synonyms

DKFZp781P1796, KIAA0188, lipin 1, LPIN1, PAP1

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Validation Data Gallery

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  WB analysis using 68124-1-Ig (same clone as 68124-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68124-1-Ig (LPIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68124-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skeletal muscle using 68124-1-Ig (same clone as 68124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68124-1-Ig (LPIN1 antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68124-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skeletal muscle using 68124-1-Ig (same clone as 68124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68124-1-Ig (LPIN1 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68124-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68124-1-Ig (same clone as 68124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68124-1-Ig (LPIN1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68124-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68124-1-Ig (same clone as 68124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68124-1-Ig (LPIN1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68124-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 68124-1-Ig (same clone as 68124-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using LPIN1 antibody (68124-1-Ig, Clone: 3G11B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68124-1-PBS in a different storage buffer formulation.

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Product Information

68124-1-PBS targets LPIN1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: LPIN1 fusion protein Ag25813
• Full Name: lipin 1
• Calculated Molecular Weight: 890 aa, 99 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC030537
• Gene Symbol: LPIN1
• Gene ID (NCBI): 23175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LPIN1, also named as KIAA0188, belongs to the lipin family.It is a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels. LPIN1 has a molecular weight of about 99 kDa but predicted bands around 130 kDa.(PMID:20231281, PMID:29765047).