LPCAT1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

LPCAT1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8B6E9

Cat no : 66044-1-PBS

Synonyms

Acetyl-CoA:lyso-PAF acetyltransferase, 8B6E9, 1-alkylglycerophosphocholine O-acetyltransferase, 1-alkenylglycerophosphocholine O-acyltransferase, 1-acylglycerophosphocholine O-acyltransferase

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Validation Data Gallery

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  WB analysis using 66044-1-Ig (same clone as 66044-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66044-1-Ig (same clone as 66044-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66044-1-Ig (same clone as 66044-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 66044-1-Ig (same clone as 66044-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-12 using 66044-1-Ig (same clone as 66044-1-PBS)

  PC-12, C6, Raw264.7, NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse lung using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using LPCAT1 antibody (66044-1-Ig, Clone: 8B6E9 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), SFTPC antibody (10774-1-AP, green). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LPCAT1 antibody (66044-1-Ig, Clone: 8B6E9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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Product Information

66044-1-PBS targets LPCAT1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: LPCAT1 fusion protein Ag9060
• Full Name: lysophosphatidylcholine acyltransferase 1
• Calculated Molecular Weight: 534 aa, 59 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC020166
• Gene Symbol: LPCAT1
• Gene ID (NCBI): 79888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LPCAT1, also named as AYTL2, PFAAP3 and LysoPAFAT, belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. It is a key enzyme for remodeling phospholipids, including phosphatidylcholine. The expression level of LPCAT1 is able to differentiate prostate cancer from noncancerous prostatic changes, and correlates to the tumor grade of prostate cancer. LPCAT1 possesses both acyltransferase and acetyltransferase activities. It mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC).