LPCAT1 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 66044-1-Ig (same clone as 66044-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66044-1-Ig (same clone as 66044-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 66044-1-Ig (same clone as 66044-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-12 using 66044-1-Ig (same clone as 66044-1-PBS)

  PC-12, C6, Raw264.7, NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66044-1-Ig (same clone as 66044-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse lung using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using LPCAT1 antibody (66044-1-Ig, Clone: 8B6E9 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), SFTPC antibody (10774-1-AP, green). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LPCAT1 antibody (66044-1-Ig, Clone: 8B6E9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50776-2

  Cytometric bead array standard curve of MP50776-2, LPCAT1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66044-1-PBS. Detection antibody: 66044-3-PBS. Standard:Ag9060. Range: 0.391-25 ng/mL.

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  Cytometric bead array standard curve of MP50776-3

  Cytometric bead array standard curve of MP50776-3, LPCAT1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66044-1-PBS. Detection antibody: 66044-2-PBS. Standard:Ag9060. Range: 0.391-100 ng/mL.

• Featured Product
• KD/KO Validated

LPCAT1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Cat No. 66044-1-PBS

Clone No.8B6E9

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

Acetyl-CoA:lyso-PAF acetyltransferase, 8B6E9, 1-alkylglycerophosphocholine O-acetyltransferase, 1-alkenylglycerophosphocholine O-acyltransferase, 1-acylglycerophosphocholine O-acyltransferase

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Product Information

66044-1-PBS targets LPCAT1 as part of a matched antibody pair:

MP50776-2: 66044-1-PBS capture and 66044-3-PBS detection (validated in Cytometric bead array)

MP50776-3: 66044-1-PBS capture and 66044-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: LPCAT1 fusion protein Ag9060
• Full Name: lysophosphatidylcholine acyltransferase 1
• Calculated Molecular Weight: 534 aa, 59 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC020166
• Gene Symbol: LPCAT1
• Gene ID (NCBI): 79888
• RRID: AB_11045658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NF37
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

LPCAT1, also named as AYTL2, PFAAP3 and LysoPAFAT, belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. It is a key enzyme for remodeling phospholipids, including phosphatidylcholine. The expression level of LPCAT1 is able to differentiate prostate cancer from noncancerous prostatic changes, and correlates to the tumor grade of prostate cancer. LPCAT1 possesses both acyltransferase and acetyltransferase activities. It mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC).