HSL Recombinant antibody, PBS Only (Detector)

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  WB analysis of human testis using 84540-1-RR (same clone as 84540-1-PBS)

  Human testis tissue were subjected to SDS PAGE followed by western blot with 84540-1-RR (HSL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

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  WB analysis using 84540-1-RR (same clone as 84540-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84540-1-RR (HSL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01374-1

  Sandwich ELISA standard curve of MP01374-1, Human HSL Recombinant Matched Antibody Pair - PBS only. 84540-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag11360. 84540-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP01374-1

  The mean HSL concentration was determined to be 3.58 ng/mL in 3T3-L1 cell extract based on a 1.30 mg/mL extract load and 1.80 ng/mL in NIH/3T3 cell extract based on a 5.90 mg/mL extract load.

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  IHC staining of mouse testis using 84540-1-RR (same clone as 84540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84540-1-RR (LIPE antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 84540-1-RR (same clone as 84540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84540-1-RR (LIPE antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brown adipose using 84540-1-RR (same clone as 84540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 84540-1-RR (HSL antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brown adipose using 84540-1-RR (same clone as 84540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 84540-1-RR (HSL antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01374-1

  Cytometric bead array standard curve of MP01374-1, HSL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84540-3-PBS. Detection antibody: 84540-1-PBS. Standard: Ag11360. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84540-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84540-1-RR against Human HSL were performed. The affinity constant is below 1 pM.

HSL Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84540-1-PBS

Clone No.241875B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

LIPE, LHS, Hormone-sensitive lipase, EC:3.1.1.79, EC:3.1.1.23

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Product Information

84540-1-PBS targets HSL as part of a matched antibody pair:

MP01374-1: 84540-3-PBS capture and 84540-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HSL fusion protein Ag11360
• Full Name: lipase, hormone-sensitive
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC070041
• Gene Symbol: HSL
• Gene ID (NCBI): 3991
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q05469
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Hormone sensitive lipase (HSL) belongs to the 'GDXG' lipolytic enzyme family. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. HSL has two isoforms with the molecular mass of 117 kDa and 84 kDa.