LIMK1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

LIMK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B11E9

Cat no : 67974-1-PBS

Synonyms

LIMK-1, LIMK 1, LIMK, LIM domain kinase 1, EC:2.7.11.1

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Validation Data Gallery

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  WB analysis using 67974-1-Ig (same clone as 67974-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67974-1-Ig (LIMK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67974-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67974-1-Ig (same clone as 67974-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67974-1-Ig (LIMK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67974-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67974-1-Ig (same clone as 67974-1-PBS)

  WB result of LIMK1 antibody (67974-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIMK1 transfected HeLa cells. This data was developed using the same antibody clone with 67974-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67974-1-Ig (same clone as 67974-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67974-1-Ig (LIMK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67974-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 67974-1-Ig (same clone as 67974-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67974-1-Ig (LIMK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67974-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 67974-1-Ig (same clone as 67974-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67974-1-Ig (LIMK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67974-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 67974-1-Ig (same clone as 67974-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67974-1-Ig (LIMK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67974-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67974-1-Ig (same clone as 67974-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LIMK1 antibody (67974-1-Ig, Clone: 2B11E9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67974-1-PBS in a different storage buffer formulation.

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Product Information

67974-1-PBS targets LIMK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: LIMK1 fusion protein Ag28862
• Full Name: LIM domain kinase 1
• Calculated Molecular Weight: 647 aa, 73 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC152982
• Gene Symbol: LIMK1
• Gene ID (NCBI): 3984
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LIMK1,also named LIMK, belongs to the protein kinase superfamily and TKL Ser/Thr protein kinase family. LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. LIMK1 may be involved in brain development. The antibody has no cross-reaction with LIMK2.