LIG4 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

LIG4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12695-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse testis using 12695-1-AP (same clone as 12695-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 12695-1-AP (same clone as 12695-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 12695-1-AP (same clone as 12695-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 12695-1-AP (same clone as 12695-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12695-1-AP (same clone as 12695-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12695-1-AP (same clone as 12695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12695-1-AP (LIG4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12695-1-AP (same clone as 12695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 12695-1-AP (LIG4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12695-1-AP (same clone as 12695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12695-1-AP (LIG4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

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Product Information

12695-1-PBS targets LIG4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIG4 fusion protein Ag3385
• Full Name: ligase IV, DNA, ATP-dependent
• Calculated Molecular Weight: 911 aa, 104 kDa
• Observed Molecular Weight: 100-104 kDa
• GenBank Accession Number: BC037491
• Gene Symbol: LIG4
• Gene ID (NCBI): 3981
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.