LIG4 Polyclonal antibody

• Featured Product
• KD/KO Validated

LIG4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 12695-1-AP

Synonyms

DNA ligase 4, DNA ligase IV, LIG4, ligase IV, DNA, ATP dependent

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Validation Data Gallery

• 

  WB analysis of mouse testis using 12695-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12695-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 12695-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse liver using 12695-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12695-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 12695-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12695-1-AP (LIG4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 12695-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 12695-1-AP (LIG4 Antibody) at dilution of 1:50.

• 

  IHC staining of human prostate cancer using 12695-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12695-1-AP (LIG4 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse liver tissue, HepG2 cells, HeLa cells, rat testis tissue
• Positive IHC detected in: human prostate cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IF: See 2 publications below

Product Information

12695-1-AP targets LIG4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIG4 fusion protein Ag3385
• Full Name: ligase IV, DNA, ATP-dependent
• Calculated Molecular Weight: 911 aa, 104 kDa
• Observed Molecular Weight: 100-104 kDa
• GenBank Accession Number: BC037491
• Gene Symbol: LIG4
• Gene ID (NCBI): 3981
• RRID: AB_2136253
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.

Publications

Species	Application	Title
human	WB	Cell Host Microbe; Cellular DNA ligase I is recruited to cytoplasmic vaccinia virus factories and masks the role of the vaccinia ligase in viral DNA replication.; Authors - Paran Nir N ; KD Validated
human	WB	Nat Commun; Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.; Authors - Guijie Guo
human	IF	J Allergy Clin Immunol; XRCC4 deficiency in human subjects causes a marked neurological phenotype but no overt immunodeficiency.; Authors - Chaowan Guo
mouse	WB	Nat Commun; Rap1 regulates hematopoietic stem cell survival and affects oncogenesis and response to chemotherapy.; Authors - Ekta Khattar
human	WB	Nat Commun; Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway.; Authors - Mengtan Xing
human	WB	Cancer Res; Inhibition of ATM Induces Hypersensitivity to Proton Irradiation by Upregulating Toxic End Joining.; Authors - Qin Zhou