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  • KD/KO Validated

LIG4 Polyclonal antibody

LIG4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12695-1-AP


DNA ligase 4, DNA ligase IV, LIG4, ligase IV, DNA, ATP dependent

Tested Applications

Positive WB detected inmouse testis tissue, mouse liver tissue, HepG2 cells, HeLa cells, rat testis tissue
Positive IHC detected inhuman prostate cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12695-1-AP targets LIG4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIG4 fusion protein Ag3385
Full Name ligase IV, DNA, ATP-dependent
Calculated Molecular Weight 911 aa, 104 kDa
Observed Molecular Weight 100-104 kDa
GenBank Accession NumberBC037491
Gene Symbol LIG4
Gene ID (NCBI) 3981
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.


Product Specific Protocols
WB protocol for LIG4 antibody 12695-1-APDownload protocol
IHC protocol for LIG4 antibody 12695-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Host Microbe

Cellular DNA ligase I is recruited to cytoplasmic vaccinia virus factories and masks the role of the vaccinia ligase in viral DNA replication.

Authors - Paran Nir N
  • KD Validated

Nat Commun

Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.

Authors - Guijie Guo

J Allergy Clin Immunol

XRCC4 deficiency in human subjects causes a marked neurological phenotype but no overt immunodeficiency.

Authors - Chaowan Guo

Nat Commun

Rap1 regulates hematopoietic stem cell survival and affects oncogenesis and response to chemotherapy.

Authors - Ekta Khattar

Nat Commun

Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway.

Authors - Mengtan Xing

Cancer Res

Inhibition of ATM Induces Hypersensitivity to Proton Irradiation by Upregulating Toxic End Joining.

Authors - Qin Zhou