LIG3 Monoclonal antibody, PBS Only (Capture)

LIG3 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10B3

Cat no : 68810-1-PBS

Synonyms

ligase III, DNA, ATP dependent, LIG2, EC:6.5.1.1, DNA ligase III, DNA ligase 3

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Validation Data Gallery

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  WB analysis using 68810-1-Ig (same clone as 68810-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68810-1-Ig (LIG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68810-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 68810-1-Ig (same clone as 68810-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68810-1-Ig (LIG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68810-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50683-2

  Cytometric bead array standard curve of MP50683-2, LIG3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68810-1-PBS. Detection antibody: 68810-3-PBS. Standard:Ag31484. Range: 1.563-100 ng/mL.

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  Cytometric bead array standard curve of MP50683-3

  Cytometric bead array standard curve of MP50683-3, LIG3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68810-1-PBS. Detection antibody: 68810-2-PBS. Standard:null. Range: null.

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Product Information

68810-1-PBS targets LIG3 as part of a matched antibody pair:

MP50683-2: 68810-1-PBS capture and 68810-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LIG3 fusion protein Ag31484
• Full Name: ligase III, DNA, ATP-dependent
• Calculated Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC068005
• Gene Symbol: LIG3
• Gene ID (NCBI): 3980
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.