DNA ligase III/LIG3 Monoclonal antibody, PBS Only (Capture)

DNA ligase III/LIG3 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10B3

Cat no : 68810-1-PBS

Synonyms

LIG3, LIG2, EC:6.5.1.1, DNA ligase III, DNA ligase 3

---

Validation Data Gallery

• 

  WB analysis using 68810-1-Ig (same clone as 68810-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68810-1-Ig (DNA ligase III/LIG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68810-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 68810-1-Ig (same clone as 68810-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68810-1-Ig (LIG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68810-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50683-2

  Cytometric bead array standard curve of MP50683-2, LIG3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68810-1-PBS. Detection antibody: 68810-3-PBS. Standard:Ag31484. Range: 1.563-100 ng/mL.

• 

  Cytometric bead array standard curve of MP50683-3

  Cytometric bead array standard curve of MP50683-3, LIG3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68810-1-PBS. Detection antibody: 68810-2-PBS. Standard:Ag31484. Range: 1.563-100 ng/mL.

---

Product Information

68810-1-PBS targets DNA ligase III/LIG3 as part of a matched antibody pair:

MP50683-2: 68810-1-PBS capture and 68810-3-PBS detection (validated in Cytometric bead array)

MP50683-3: 68810-1-PBS capture and 68810-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DNA ligase III/LIG3 fusion protein Ag31484
• Full Name: ligase III, DNA, ATP-dependent
• Calculated Molecular Weight: 100-110 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC068005
• Gene Symbol: LIG3
• Gene ID (NCBI): 3980
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DNA ligase III (DNA ligase 3) is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.