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LIG3 Monoclonal antibody

LIG3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B10B3

Cat no : 68810-1-Ig

Synonyms

DNA ligase 3, DNA ligase III, LIG2, LIG3, ligase III, DNA, ATP dependent

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LIG3 Monoclonal antibody (68810-1-Ig)

    WB analysis using 68810-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68810-1-Ig (LIG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using LIG3 Monoclonal antibody (68810-1-Ig)

    WB analysis of HSC-T6 using 68810-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68810-1-Ig (LIG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68810-1-Ig targets LIG3 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LIG3 fusion protein Ag31484
Full Name ligase III, DNA, ATP-dependent
Calculated Molecular Weight 100-110 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC068005
Gene Symbol LIG3
Gene ID (NCBI) 3980
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA ligase 3 is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.

Protocols

Product Specific Protocols
WB protocol for LIG3 antibody 68810-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols