LIF Recombinant antibody, PBS Only (Capture/Detector)

LIF Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241519A6

Cat no : 84232-2-PBS

Synonyms

Leukemia inhibitory factor, Melanoma-derived LPL inhibitor, Differentiation-stimulating factor, 241519A6

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01130-4

  Sandwich ELISA standard curve of MP01130-4, Human LIF Recombinant Matched Antibody Pair - PBS only. 84232-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0111. 84232-5-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

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  Sample test of MP01130-4

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 4 days. The mean LIF concentration was determined to be 1.4 pg/mL in unstimulated PBMC supernatant, 2,1724.1 pg/mL in PHA stimulated PBMC supernatant.

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  Sample test of MP01130-4

  Serum of eight individual healthy human donors was measured. The LIF concentration of detected samples was determined to be 3.0 pg/mL with a range of 1.5-5.2 pg/mL.

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  Cytometric bead array standard curve of MP01130-1

  Cytometric bead array standard curve of MP01130-1, LIF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84232-3-PBS. Detection antibody: 84232-2-PBS. Standard: Eg0111. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP01130-2

  Cytometric bead array standard curve of MP01130-2, LIF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84232-4-PBS. Detection antibody: 84232-2-PBS. Standard: Eg0111. Range: 0.625-80 ng/mL.

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Product Information

84232-2-PBS targets LIF as part of a matched antibody pair:

MP01130-1: 84232-3-PBS capture and 84232-2-PBS detection (validated in Cytometric bead array)

MP01130-2: 84232-4-PBS capture and 84232-2-PBS detection (validated in Cytometric bead array)

MP01130-4: 84232-2-PBS capture and 84232-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: leukemia inhibitory factor (cholinergic differentiation factor)
• Calculated Molecular Weight: 202 aa, 22 kDa
• GenBank Accession Number: BC093733
• Gene Symbol: LIF
• Gene ID (NCBI): 3976
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.