LGALS3BP Monoclonal antibody, PBS Only

LGALS3BP Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D8E11

Cat no : 60066-1-PBS

Synonyms

90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K

---

Validation Data Gallery

• 

  WB analysis of human blood using 60066-1-Ig (same clone as 60066-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60066-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human blood using 60066-1-Ig (same clone as 60066-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60066-1-PBS in a different storage buffer formulation.

• 

  IP experiment of human plasma using 60066-1-Ig (same clone as 60066-1-PBS)

  IP result of anti-LGALS3BP (IP:60066-1-Ig, 5ug; Detection:60066-1-Ig 1:1000) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 60066-1-PBS in a different storage buffer formulation.

• 

  IP experiment of human plasma using 60066-1-Ig (same clone as 60066-1-PBS)

  IP result of anti-LGALS3BP (IP:60066-1-Ig, 5ug; Detection:60066-1-Ig 1:300) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 60066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60066-1-Ig (same clone as 60066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60066-1-Ig (same clone as 60066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60066-1-Ig (same clone as 60066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60066-1-Ig (same clone as 60066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 60066-1-Ig (same clone as 60066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 60066-1-Ig (same clone as 60066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60066-1-Ig (same clone as 60066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60066-1-Ig (same clone as 60066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 60066-1-Ig (same clone as 60066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 60066-1-Ig (same clone as 60066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60066-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 60066-1-Ig (same clone as 60066-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LGALS3BP antibody (60066-1-Ig, Clone: 2D8E11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60066-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60066-1-PBS targets LGALS3BP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: LGALS3BP fusion protein Ag0294
• Full Name: lectin, galactoside-binding, soluble, 3 binding protein
• Calculated Molecular Weight: 585 aa, 65 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC002403
• Gene Symbol: LGALS3BP
• Gene ID (NCBI): 3959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ). LGALS3BP has a predicted mass of ∼65 kDa. It is heavily glycosylated and shows an apparent molecular weight of 90 kDa on SDS-PAGE.