LGALS3BP Polyclonal antibody, PBS Only

LGALS3BP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23307-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HepG2 using 23307-1-AP (same clone as 23307-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23307-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23307-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 23307-1-AP (same clone as 23307-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23307-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23307-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 23307-1-AP (same clone as 23307-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 23307-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 23307-1-AP (same clone as 23307-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23307-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 23307-1-AP (same clone as 23307-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23307-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 23307-1-AP (same clone as 23307-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 23307-1-AP (LGALS3BP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23307-1-PBS in a different storage buffer formulation.

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Product Information

23307-1-PBS targets LGALS3BP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LGALS3BP fusion protein Ag19700
• Full Name: lectin, galactoside-binding, soluble, 3 binding protein
• Calculated Molecular Weight: 585 aa, 65 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC002403
• Gene Symbol: LGALS3BP
• Gene ID (NCBI): 3959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ).