CoraLite® Plus 488-conjugated LETM1 Polyclonal antibody

• Featured Product
• KD/KO Validated

LETM1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16024

Synonyms

LETM1

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Validation Data Gallery

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  IF Staining of HeLa using CL488-16024

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 LETM1 antibody (CL488-16024) at dilution of 1:800.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-16024 targets LETM1 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LETM1 fusion protein Ag8861
• Full Name: leucine zipper-EF-hand containing transmembrane protein 1
• Calculated Molecular Weight: 739 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC021208
• Gene Symbol: LETM1
• Gene ID (NCBI): 3954
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LETM1 is a mitochondrial inner membrane protein. It functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. It served as an anchor protein for complex formation between mitochondria and ribosome, and regulated mitochondrial biogenesis. It was also identified inWolf-Hirschhorn syndrome, and was deleted in nearly all patients with the syndrome. A recent study determined that adenovirus-mediated LETM1 may provide a useful target for designing lung tumor prevention and treatment.