LDLR Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

LDLR Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A2A3

Cat no : 66414-1-PBS

Synonyms

LDL receptor, FHC, FH, 1A2A3

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Validation Data Gallery

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  WB analysis of HeLa using 66414-1-Ig (same clone as 66414-1-PBS)

  WB result of LDLR antibody (66414-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LDLR transfected HeLa cells. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66414-1-Ig (same clone as 66414-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66414-1-Ig (same clone as 66414-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66414-1-Ig (same clone as 66414-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66414-1-Ig (same clone as 66414-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66414-1-Ig (same clone as 66414-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66414-1-Ig (LDLR Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66414-1-Ig (same clone as 66414-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66414-1-Ig (LDLR Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

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66414-1-Ig

Product Information

66414-1-PBS targets LDLR in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LDLR fusion protein Ag1236
• Full Name: low density lipoprotein receptor
• Calculated Molecular Weight: 95 kDa
• Observed Molecular Weight: 120 kDa, 160 kDa
• GenBank Accession Number: BC014514
• Gene Symbol: LDLR
• Gene ID (NCBI): 3949
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.