LDLR Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

LDLR Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10785-1-PBS

Synonyms

LDL receptor, FHC, FH

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Validation Data Gallery

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  WB analysis using 10785-1-AP (same clone as 10785-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10785-1-AP (same clone as 10785-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 10785-1-AP (same clone as 10785-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 10785-1-AP (same clone as 10785-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 10785-1-AP (same clone as 10785-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 10785-1-AP (same clone as 10785-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 10785-1-AP (same clone as 10785-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 10785-1-AP (same clone as 10785-1-PBS)

  IP result of anti-LDLR (IP:10785-1-AP, 5ug; Detection:10785-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10785-1-AP (LDLR antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10785-1-AP (LDLR antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10785-1-AP (LDLR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10785-1-AP (LDLR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 10785-1-AP (same clone as 10785-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LDLR antibody (10785-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Beta Actin antibody (66009-1-Ig, Clone: 2D4H5, red). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

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Product Information

10785-1-PBS targets LDLR in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LDLR fusion protein Ag1236
• Full Name: low density lipoprotein receptor
• Calculated Molecular Weight: 95 kDa
• Observed Molecular Weight: 100-160 kDa
• GenBank Accession Number: BC014514
• Gene Symbol: LDLR
• Gene ID (NCBI): 3949
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.