CoraLite®594-conjugated LDHB Monoclonal antibody

LDHB Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5E9G1

Cat no : CL594-66425

Synonyms

lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, TRG 5

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Validation Data Gallery

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  IF Staining of HeLa using CL594-66425

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 LDHB antibody (CL594-66425, Clone: 5E9G1 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66425 targets LDHB in IF/ICC applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LDHB fusion protein Ag6605
• Full Name: lactate dehydrogenase B
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC002362
• Gene Symbol: LDHB
• Gene ID (NCBI): 3945
• RRID: AB_2919989
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway.It is glycolytic enzymes and it is positively regulated by mTOR.