LDHA-Specific Recombinant antibody, PBS Only

LDHA-Specific Uni-rAb^TM Recombinant Antibody for IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240885F4

Cat no : 84198-1-PBS

Synonyms

LDHA, LDH A, lactate dehydrogenase A, EC:1.1.1.27, Cell proliferation-inducing gene 19 protein

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Validation Data Gallery

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  IF Staining of HepG2 using 84198-1-RR (same clone as 84198-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LDHA-Specific antibody (84198-1-RR, Clone: 240885F4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84198-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84198-1-PBS

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LDHA-Specific antibody (84198-1-PBS, Clone: 240885F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84198-1-PBS targets LDHA-Specific in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: lactate dehydrogenase A
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: NM_005566
• Gene Symbol: LDHA
• Gene ID (NCBI): 3939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies.