CoraLite® Plus 488-conjugated LC3 Recombinant antibody

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  IF Staining of HepG2 using CL488-81004

  Immunofluorescent analysis of (-20°C Methanol) fixed Chloroquine treated HepG2 cells using CoraLite® Plus 488 LC3 antibody (CL488-81004, Clone: 5P12 ) at dilution of 1:500.

• Featured Product
• KD/KO Validated

LC3 Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-81004

Clone No.5P12

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat, Pig

Applications

IF/ICC

Synonyms

LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3

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Tested Applications

• Positive IF/ICC detected in: Chloroquine treated HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-81004 targets LC3 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LC3 fusion protein Ag6144
• Full Name: microtubule-associated protein 1 light chain 3 beta
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 14-18 kDa
• GenBank Accession Number: BC067797
• Gene Symbol: LC3B
• Gene ID (NCBI): 81631
• ENSEMBL Gene ID: ENSG00000140941
• RRID: AB_3084496
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9GZQ8
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane.