LC3 Polyclonal antibody, PBS Only

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  WB analysis using 14600-1-AP (same clone as 14600-1-PBS)

  Non-treated and Chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

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  WB analysis using 14600-1-AP (same clone as 14600-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14600-1-AP (LC3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14600-1-AP (same clone as 14600-1-PBS)

  WB result of LC3 antibody (14600-1-AP; 1:6000; room temperature for 1.5 hours) with negative Control and LC3 knockout HeLa cells. This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14600-1-AP (same clone as 14600-1-PBS)

  WB result of LC3 antibody (14600-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LC3 transfected HeLa cells. This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14600-1-AP (same clone as 14600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 14600-1-AP (same clone as 14600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 14600-1-AP (same clone as 14600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14600-1-AP (same clone as 14600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14600-1-AP (same clone as 14600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 14600-1-AP (same clone as 14600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14600-1-AP (same clone as 14600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14600-1-AP (same clone as 14600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14600-1-AP (LC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14600-1-AP (same clone as 14600-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using LC3 antibody (14600-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14600-1-AP (same clone as 14600-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed CQ treated HepG2 cells using 14600-1-AP (LC3 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14600-1-AP (same clone as 14600-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed CQ treated HepG2 cells using 14600-1-AP (LC3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 14600-1-AP (same clone as 14600-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human LC3 (14600-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 14600-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

LC3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 14600-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

MAP1LC3B, ATG8F, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, MAP1 light chain 3-like protein 2

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Product Information

14600-1-PBS targets LC3 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LC3 fusion protein Ag6144
• Full Name: microtubule-associated protein 1 light chain 3 beta
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 14-18 kDa
• GenBank Accession Number: BC067797
• Gene Symbol: LC3B
• Gene ID (NCBI): 81631
• ENSEMBL Gene ID: ENSG00000140941
• RRID: AB_2137737
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9GZQ8
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.