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LBR Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.195-12.5 ng/mL

Cat No : MP01373-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01373-3, Human LBR Recombinant Matched Antibody Pair - PBS only. 84522-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3088. 84522-5-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL.

    Sandwich ELISA standard curve of MP01373-3

    Sandwich ELISA standard curve of MP01373-3, Human LBR Recombinant Matched Antibody Pair - PBS only. 84522-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3088. 84522-5-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL.

  • The mean LBR concentration was determined to be 69.49 ng/mL in K-562 cell extract based on a 2.40 mg/mL extract load, 84.74 ng/mL in Jurkat cell extract based on a 1.50 mg/mL extract load and 200.46 ng/mL in A431 cell extract based on a 1.50 mg/mL extract load.

    Sample test of MP01373-3

    The mean LBR concentration was determined to be 69.49 ng/mL in K-562 cell extract based on a 2.40 mg/mL extract load, 84.74 ng/mL in Jurkat cell extract based on a 1.50 mg/mL extract load and 200.46 ng/mL in A431 cell extract based on a 1.50 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01373-3 targets LBR in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.195-12.5 ng/mL (Sandwich ELISA);

Capture antibody: LBR Recombinant antibody, PBS Only (Capture) 84522-1-PBS (241864B4). 100 μg. Concentration 1 mgl/ml.

Detection antibody: LBR Recombinant antibody, PBS Only (Detector) 84522-5-PBS (241864E11). 100 μg. Concentration 1 mgl/ml.

Alternative LBR matched antibody pairs: MP01373-1, MP01373-2

Matched pair protein standard: Ag3088

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84522-1-PBS
Tested Reactivity human
Immunogen LBR fusion protein Ag3088
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lamin B receptor
Synonyms Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta, 241864B4
GenBank accession numberBC020079
Gene symbol LBR
UniprotQ14739
Gene ID (NCBI) 3930
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84522-5-PBS
Tested Reactivity human
Immunogen LBR fusion protein Ag3088
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lamin B receptor
Synonyms
GenBank accession numberBC020079
Gene symbol LBR
UniprotQ14739
Gene ID (NCBI) 3930
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.