LBR Monoclonal antibody, PBS Only

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  WB analysis using 68378-1-Ig (same clone as 68378-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68378-1-Ig (LBR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68378-1-Ig (same clone as 68378-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68378-1-Ig (LBR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68378-1-Ig (same clone as 68378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68378-1-Ig (LBR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68378-1-Ig (same clone as 68378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68378-1-Ig (LBR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68378-1-Ig (same clone as 68378-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LBR antibody (68378-1-Ig, Clone: 1A1B7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68378-1-PBS in a different storage buffer formulation.

LBR Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 68378-1-PBS

Clone No.1A1B7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta, 1A1B7

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Product Information

68378-1-PBS targets LBR in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LBR fusion protein Ag25601
• Full Name: lamin B receptor
• Calculated Molecular Weight: 615 aa, 71 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC020079
• Gene Symbol: LBR
• Gene ID (NCBI): 3930
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14739
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).