LBR Monoclonal antibody

LBR Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A1B7

Cat no : 68378-1-Ig

Synonyms

Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta, 1A1B7

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Validation Data Gallery

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  WB analysis using 68378-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68378-1-Ig (LBR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68378-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68378-1-Ig (LBR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 68378-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68378-1-Ig (LBR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 68378-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68378-1-Ig (LBR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 68378-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LBR antibody (68378-1-Ig, Clone: 1A1B7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

• Positive WB detected in: U2OS cells, HSC-T6 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, ROS1728 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68378-1-Ig targets LBR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LBR fusion protein Ag25601
• Full Name: lamin B receptor
• Calculated Molecular Weight: 615 aa, 71 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC020079
• Gene Symbol: LBR
• Gene ID (NCBI): 3930
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).