LBP Monoclonal antibody, PBS Only

LBP Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A7E3

Cat no : 66181-1-PBS

Synonyms

LBP

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Validation Data Gallery

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  WB analysis of Jurkat using 66181-1-Ig (same clone as 66181-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66181-1-Ig (LBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66181-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66181-1-Ig (same clone as 66181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66181-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66181-1-Ig (same clone as 66181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66181-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66181-1-Ig (same clone as 66181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66181-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66181-1-Ig (same clone as 66181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66181-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66181-1-Ig (same clone as 66181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66181-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66181-1-Ig (same clone as 66181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66181-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66181-1-PBS targets LBP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: LBP fusion protein Ag13485
• Full Name: lipopolysaccharide binding protein
• Calculated Molecular Weight: 481 aa, 53 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC022256
• Gene Symbol: LBP
• Gene ID (NCBI): 3929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LBP (lipopolysaccharide-binding protein) is an acute-phase protein, predominantly synthesized by hepatocytes. It is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. (PMID: 9665271; 11598069)