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LBP Monoclonal antibody

LBP Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A7E3

Cat no : 66181-1-Ig

Synonyms

LBP

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using LBP Monoclonal antibody (66181-1-Ig)

    WB analysis of Jurkat using 66181-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66181-1-Ig (LBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using LBP Monoclonal antibody (66181-1-Ig)

    IHC staining of human liver using 66181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using LBP Monoclonal antibody (66181-1-Ig)

    IHC staining of human liver using 66181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LBP Monoclonal antibody (66181-1-Ig)

    IHC staining of human liver cancer using 66181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LBP Monoclonal antibody (66181-1-Ig)

    IHC staining of human liver cancer using 66181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LBP Monoclonal antibody (66181-1-Ig)

    IHC staining of human kidney using 66181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LBP Monoclonal antibody (66181-1-Ig)

    IHC staining of human kidney using 66181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66181-1-Ig targets LBP in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen LBP fusion protein Ag13485
Full Name lipopolysaccharide binding protein
Calculated Molecular Weight 481 aa, 53 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC022256
Gene Symbol LBP
Gene ID (NCBI) 3929
RRIDAB_2881576
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LBP (lipopolysaccharide-binding protein) is an acute-phase protein, predominantly synthesized by hepatocytes. It is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. (PMID: 9665271; 11598069)

Protocols

Product Specific Protocols
WB protocol for LBP antibody 66181-1-IgDownload protocol
IHC protocol for LBP antibody 66181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IF

Nat Commun

Lipopolysaccharide binding protein resists hepatic oxidative stress by regulating lipid droplet homeostasis

Authors - Qilun Zhang
View Article