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CoraLite® Plus 488-conjugated LASP1 Monoclonal antibody

LASP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G4B6

Cat no : CL488-68080

Synonyms

MLN50, LIM and SH3 protein 1, LASP-1, 1G4B6

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated LASP1 Monoclonal ant (CL488-68080)

    IF Staining of A549 using CL488-68080

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 LASP1 antibody (CL488-68080, Clone: 1G4B6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated LASP1 Monoclonal ant (CL488-68080)

    IF Staining of A549 using CL488-68080

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 LASP1 antibody (CL488-68080, Clone: 1G4B6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A549 cells using CoraLite® Plus 488-conjugated LASP1 Monoclonal ant (CL488-68080)

    FC experiment of A549 using CL488-68080

    1X10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human LASP1 (CL488-68080, Clone:1G4B6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68080 targets LASP1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LASP1 fusion protein Ag18101
Full Name LIM and SH3 protein 1
Calculated Molecular Weight 30 kDa
Observed Molecular Weight35-38 kDa
GenBank Accession NumberBC012460
Gene Symbol LASP1
Gene ID (NCBI) 3927
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 LASP1 antibody CL488-68080Download protocol
FC protocol for CL Plus 488 LASP1 antibody CL488-68080Download protocol
Standard Protocols
Click here to view our Standard Protocols