LARP2 Recombinant antibody

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  IHC staining of mouse testis using 84898-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84898-3-RR (LARP2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 84898-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84898-3-RR (LARP2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 84898-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using LARP2 antibody (84898-3-RR, Clone: 242486E9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 84898-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug LARP2 Recombinant antibody (84898-3-RR, Clone:242486E9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84898-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84898-3-RR against Human LARP2 were performed. The affinity constant is below 1 pM.

LARP2 {ptg:Brand} Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Cat No. 84898-3-RR

Clone No.242486E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

LARP1B, La-related protein 2, La-related protein 1B, La related protein 2, La related protein 1B

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Tested Applications

• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84898-3-RR targets LARP2 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LARP2 fusion protein Ag11043
• Full Name: La ribonucleoprotein domain family, member 2
• Calculated Molecular Weight: 201 aa, 24 kDa
• GenBank Accession Number: BC030516
• Gene Symbol: LARP2
• Gene ID (NCBI): 55132
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q659C4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.