CoraLite®555-conjugated LARP1 Monoclonal antibody

LARP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2E9F2

Cat no : CL555-67810

Synonyms

LARP, La-related protein 1, La related protein 1, KIAA0731, 2E9F2

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Validation Data Gallery

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  IF Staining of HepG2 using CL555-67810

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 LARP1 antibody (CL555-67810, Clone: 2E9F2 ) at dilution of 1:400.

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  FC experiment of HepG2 using CL555-67810

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human LARP1 (CL555-67810, Clone:2E9F2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-67810 targets LARP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LARP1 fusion protein Ag29993
• Full Name: La ribonucleoprotein domain family, member 1
• Calculated Molecular Weight: 1096 aa, 124 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC033856
• Gene Symbol: LARP1
• Gene ID (NCBI): 23367
• RRID: AB_2919705
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LARP1, full named as La-related Protein 1, is an oncogenic RNA-binding protein required for ribosome biogenesis and cancer cell survival. (PMID: 32286153)It's has been proposed to function downstream of mTORC1 to regulate the translation of 5'TOP mRNAs such as those encoding ribosome proteins (RP). (PMID: 28650797) LARP1 has 2 isoforms with MW of 123-150KDa. With CL647, immunoflorecence detected location in cytoplasm.