NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 66417-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 66417-1-Ig
HepG2 cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of C2C12 using 66417-1-Ig
C2C12 cell were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
C2C12 cell were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human lung using 66417-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66417-1-Ig (LAP3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66417-1-Ig (LAP3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 66417-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66417-1-Ig (LAP3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66417-1-Ig (LAP3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 66417-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LAP3 antibody (66417-1-Ig, Clone: 1B11B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LAP3 antibody (66417-1-Ig, Clone: 1B11B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
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NIH/3T3 cells, C2C12 cell, HeLa cells, HepG2 cells
Positive IHC detected in
human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:6000
Immunohistochemistry (IHC)
IHC : 1:100-1:400
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
66417-1-Ig targets LAP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
LAP3(Leucine aminopeptidase 3) is also named as LAPEP, PEPS(peptidase S) and belongs to the peptidase M17 family. It can catalyzes the removal of unsubstituted N-terminal amino-acids from various peptides and be presumably involved in the processing end regular turnover of intracellular proteins. PEPS is found in a wide variety of tissues and cultured cells but not in red cells and skin. The enzyme can utilize several di-, tri-, and tetrapeptides as substrates and is the slowest migrating of the peptidases after electrophoresis.(PMID:689684). LAP3 has two isoform with MW of 52 and 56 kDa.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 66417-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 66417-1-Ig
HepG2 cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of C2C12 using 66417-1-Ig
C2C12 cell were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human lung using 66417-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66417-1-Ig (LAP3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 66417-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66417-1-Ig (LAP3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HeLa using 66417-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LAP3 antibody (66417-1-Ig, Clone: 1B11B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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