LAMTOR1 Polyclonal antibody

LAMTOR1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29611-1-AP

Synonyms

C11orf59, p27RF-Rho, p27RF Rho, p27Kip1-releasing factor from RhoA, Lipid raft adaptor protein p18

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Validation Data Gallery

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  WB analysis using 29611-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29611-1-AP (C11orf59,LAMTOR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-87 MG using 29611-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using LAMTOR1 antibody (29611-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, MCF-7 cells
• Positive IF/ICC detected in: U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29611-1-AP targets LAMTOR1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LAMTOR1 fusion protein Ag31215
• Full Name: chromosome 11 open reading frame 59
• Calculated Molecular Weight: 161 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC001706
• Gene Symbol: LAMTOR1
• Gene ID (NCBI): 55004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAMTOR1 (Late Endosome/Lysosome-Associated Membrane Protein 1) is a membrane protein specifically localized to the surface of late endosomes/lysosomes and is involved in lysosomal transport and maturation. LAMTOR1 is a key component of the lysosomal articulating protein complex Ragulator, which is required for the localization of the Ragulator complex to lysosomes. LAMTOR1 is a key component of the lysosomal bridging protein complex Ragulator and is required for the localization of the Ragulator complex to the lysosome. LAMTOR1 is involved in autophagy, cell growth, immune regulation, cancer development, and regulatory pathways of glucose metabolism. Recent studies have shown that in calorie restriction, lithocholic acid activates AMPK through the glucose-sensing pathway ( the lysosomal pathway ) and the body senses the metabolic signals induced by calorie restriction and exerts its role in slowing down the effects of aging and prolonging the lifespan, whereas deletion of LAMTOR1 results in the inability of AMPK to be activated in order to play its role.