Filter: AllWBIHC

WB analysis using 66301-1-Ig (same clone as 66301-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
WB analysis using 66301-1-Ig (same clone as 66301-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
WB analysis of pig liver using 66301-1-Ig (same clone as 66301-1-PBS)
pig liver tissue were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 66301-1-Ig (same clone as 66301-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66301-1-Ig (CD107b / LAMP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66301-1-Ig (CD107b / LAMP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66301-1-Ig (same clone as 66301-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

CD107b / LAMP2 Monoclonal antibody, PBS Only

CD107b / LAMP2 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66301-1-PBS

Clone No.2D3B9

Review Product

Host / Isotype

Mouse / IgA

Reactivity

human, mouse, pig

Applications

WB, IHC, ELISA

Synonyms

CD107b, LAMP2, 2D3B9, CD107 antigen-like family member B, LAMP 2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66301-1-PBS targets CD107b / LAMP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity	human, mouse, pig
Host / Isotype	Mouse / IgA
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag0595 Product name: Recombinant human LAMP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 255-390 aa of BC002965 Sequence: NPNTTHSTGSCRSHTALLRLNSSTIKYLDFVFAVKNENRFYLKEVNISMYLVNGSVFSIANNNLSYWDAPLGSSYMCNKEQTVSVSGAFQINTFDLRVQPFNVTQGKYSTAQECSLDDDTILIPIIVGAGLSGLII Predict reactive species
Full Name	lysosomal-associated membrane protein 2
Calculated Molecular Weight	45 kDa
Observed Molecular Weight	120 kDa
GenBank Accession Number	BC002965
Gene Symbol	LAMP2
Gene ID (NCBI)	3920
ENSEMBL Gene ID	ENSG00000005893
RRID	AB_2881684
Conjugate	Unconjugated
Form	Liquid
Purification Method	Thiophilic affinity chromatograph
UNIPROT ID	P13473
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides.