CD107b / LAMP2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CD107b / LAMP2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgA

Reactivity

human, mouse, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D3B9

Cat no : 66301-1-PBS

Synonyms

CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker

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Validation Data Gallery

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  WB analysis using 66301-1-Ig (same clone as 66301-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66301-1-Ig (same clone as 66301-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 66301-1-Ig (same clone as 66301-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 66301-1-Ig (same clone as 66301-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66301-1-Ig (LAMP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66301-1-Ig (same clone as 66301-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66301-1-Ig (same clone as 66301-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66301-1-Ig (LAMP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66301-1-Ig (same clone as 66301-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66301-1-Ig (CD107b / LAMP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66301-1-Ig (same clone as 66301-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66301-1-Ig (CD107b / LAMP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66301-1-Ig (same clone as 66301-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66301-1-Ig (same clone as 66301-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66301-1-Ig (same clone as 66301-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66301-1-Ig (same clone as 66301-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66301-1-Ig (same clone as 66301-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66301-1-Ig (same clone as 66301-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66301-1-Ig (LAMP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66301-1-Ig (same clone as 66301-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66301-1-Ig (LAMP2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), phalloidine stains F-actin (red). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66301-1-Ig (same clone as 66301-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66301-1-Ig (LAMP2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), phalloidine stains F-actin (red). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66301-1-Ig (same clone as 66301-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66301-1-Ig (LAMP2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

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  IF Staining of 3T3-L1 using 66301-1-Ig (same clone as 66301-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed 3T3-L1 cells using 66301-1-Ig (LAMP2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66301-1-Ig (same clone as 66301-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66301-1-Ig(LAMP2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66301-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66301-1-PBS targets CD107b / LAMP2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgA
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD107b / LAMP2 fusion protein Ag0595
• Full Name: lysosomal-associated membrane protein 2
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC002965
• Gene Symbol: LAMP2
• Gene ID (NCBI): 3920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides.