CoraLite® Plus 750-conjugated LAMB2 Recombinant antibody

LAMB2 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230143E4

Cat no : CL750-82946

Synonyms

LAMS, Laminin 3 subunit beta, Laminin 15 subunit beta, Laminin 14 subunit beta, Laminin 11 subunit beta

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Validation Data Gallery

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  WB analysis using CL750-82946

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-82946 (LAMB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using CL750-82946

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Coralite® Plus 750 Lamb2 Recombinant Antibody (CL750-82946, Clone:230143E4)(red), or 0.25 ug CoraLite® Plus 750 Rabbit IgG Isotype Control Recombinant Antibody (CL750-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-82946 targets LAMB2 in WB, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LAMB2 fusion protein Ag33468
• Full Name: laminin, beta 2 (laminin S)
• Calculated Molecular Weight: 196 kDa
• Observed Molecular Weight: 195-200 kDa
• GenBank Accession Number: NM_002292.3
• Gene Symbol: LAMB2
• Gene ID (NCBI): 3913
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LAMB2 (laminin subunit beta 2), also known as LAMS. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. This gene encodes the beta chain isoform laminin, beta 2. It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination, showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration. The calculated molecular weight of LAMB2 is 200 kDa.