CoraLite®594-conjugated LAIR1 Monoclonal antibody

LAIR1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A5D1

Cat no : CL594-67220

Synonyms

CD305, hLAIR1, LAIR 1, LAIR1

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL594-67220

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 LAIR1 antibody (CL594-67220, Clone: 1A5D1 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL594-67220

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 LAIR1 antibody (CL594-67220, Clone: 1A5D1 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67220 targets LAIR1 in IF-P applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: LAIR1 fusion protein Ag14459
• Full Name: leukocyte-associated immunoglobulin-like receptor 1
• Calculated Molecular Weight: 287 aa, 31 kDa
• GenBank Accession Number: BC027899
• Gene Symbol: LAIR1
• Gene ID (NCBI): 3903
• RRID: AB_2920100
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) is a transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs (PMID: 9285412). LAIR1 is expressed on the majority of human PBMCs, including NK, T, B, monocytes, and dendritic cells, as well as the majority of thymocytes (PMID: 10229813). It functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of NK cells, B cells and T cells. LAIR1 is a collagen receptor (PMID: 16754721). Tumor-expressed collagens can modulate immune cell function through the inhibitory collagen receptor LAIR1 (PMID: 21955987).