LACTB Monoclonal antibody, PBS Only

LACTB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C3C3

Cat no : 66785-1-PBS

Synonyms

Serine beta-lactamase-like protein LACTB, mitochondrial, MRPL56, lactamase, beta, G24, EC:3.4.-.-

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Validation Data Gallery

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  WB analysis using 66785-1-Ig (same clone as 66785-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66785-1-Ig (LACTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66785-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66785-1-Ig (same clone as 66785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66785-1-Ig (LACTB antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66785-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66785-1-Ig (same clone as 66785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66785-1-Ig (LACTB antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66785-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66785-1-Ig (same clone as 66785-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using LACTB antibody (66785-1-Ig, Clone: 3C3C3 ) at dilution of 1:1600 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66785-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66785-1-Ig (same clone as 66785-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66785-1-Ig (LACTB antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66785-1-PBS in a different storage buffer formulation.

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Product Information

66785-1-PBS targets LACTB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LACTB fusion protein Ag12233
• Full Name: lactamase, beta
• Calculated Molecular Weight: 547 aa, 61 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC067288
• Gene Symbol: LACTB
• Gene ID (NCBI): 114294
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LACTB is also named as MRPL56,whic is a component of the large subunit of the mitochondrial ribosome that is encoded by the nuclear genome.LACTB is a mammalian active-site serine protein that has evolved from a bacterial penicillin-binding protein.It promotes intramitochondrial membrane organization and micro-compartmentalization through polymerization(PMID:19858488). Northern blot analysis of mouse tissues detected expression at high levels in liver, heart, and skeletal muscle, with lower levels in kidney, testis, brain, lung, and spleen(PMID:11707067). The full-length of LACTB is about 61kDa, and a 115aa transit peptide can be removed in the mature form.