LACTB Monoclonal antibody

• Featured Product
• KD/KO Validated

LACTB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

3C3C3

Cat no : 66785-1-Ig

Synonyms

Serine beta-lactamase-like protein LACTB, mitochondrial, MRPL56, lactamase, beta, G24, EC:3.4.-.-

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Validation Data Gallery

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  WB analysis using 66785-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66785-1-Ig (LACTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66785-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66785-1-Ig (LACTB antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66785-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66785-1-Ig (LACTB antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 66785-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using LACTB antibody (66785-1-Ig, Clone: 3C3C3 ) at dilution of 1:1600 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  IF Staining of HepG2 using 66785-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66785-1-Ig (LACTB antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

66785-1-Ig targets LACTB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LACTB fusion protein Ag12233
• Full Name: lactamase, beta
• Calculated Molecular Weight: 547 aa, 61 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC067288
• Gene Symbol: LACTB
• Gene ID (NCBI): 114294
• RRID: AB_2882130
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LACTB is also named as MRPL56,whic is a component of the large subunit of the mitochondrial ribosome that is encoded by the nuclear genome.LACTB is a mammalian active-site serine protein that has evolved from a bacterial penicillin-binding protein.It promotes intramitochondrial membrane organization and micro-compartmentalization through polymerization(PMID:19858488). Northern blot analysis of mouse tissues detected expression at high levels in liver, heart, and skeletal muscle, with lower levels in kidney, testis, brain, lung, and spleen(PMID:11707067). The full-length of LACTB is about 61kDa, and a 115aa transit peptide can be removed in the mature form.

Publications

Species	Application	Title
human	WB, IHC, IF	Cancer Lett; LACTB suppresses melanoma progression by attenuating PP1A and YAP interaction.; Authors - Yawen Ma ; KD Validated
human	WB	Cancer Manag Res; MicroRNA-1276 Promotes Colon Cancer Cell Proliferation by Negatively Regulating LACTB.; Authors - Chunxiao Wang
human,mouse	WB	Mol Cell; OXCT1 functions as a succinyltransferase, contributing to hepatocellular carcinoma via succinylating LACTB; Authors - Wenhao Ma
human	WB,IP	STAR Protoc; Protocol for identifying OXCT1-mediated LACTB succinylation sites in vitro; Authors - Wenhao Ma