CoraLite® Plus 488-conjugated L2HGDH Monoclonal antibody

L2HGDH Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3D11C2

Cat no : CL488-66269

Synonyms

C14orf160, DURANIN, FLJ12618, L2HGDH

---

Validation Data Gallery

• 

  IF Staining of human breast cancer using CL488-66269

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 L2HGDH antibody (CL488-66269, Clone: 3D11C2 ) at dilution of 1:200.

---

Tested Applications

• Positive IF-P detected in: human breast cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66269 targets L2HGDH in IF-P applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: L2HGDH fusion protein Ag8382
• Full Name: L-2-hydroxyglutarate dehydrogenase
• Calculated Molecular Weight: 463aa,50 kDa; 441aa,49 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC006117
• Gene Symbol: L2HGDH
• Gene ID (NCBI): 79944
• RRID: AB_2919308
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family. The putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa. L2HGDH also can be detected as ~45kD due to the 51aa transit peptide cleaved.