CoraLite® Plus 488-conjugated L1CAM Monoclonal antibody

L1CAM Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat and More (1)

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

CloneNo.

3H7B9

Cat no : CL488-67115

Synonyms

CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1

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Validation Data Gallery

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  IF Staining of mouse brain using CL488-67115

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 L1CAM antibody (CL488-67115, Clone: 3H7B9 ) at dilution of 1:200.

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  IF Staining of mouse brain using CL488-67115

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 L1CAM antibody (CL488-67115, Clone: 3H7B9 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL488-67115 targets L1CAM in IF-P applications and shows reactivity with Human, Pig, Mouse, Rat samples.

• Tested Reactivity: Human, Pig, Mouse, Rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: L1CAM fusion protein Ag17706
• Full Name: L1 cell adhesion molecule
• Calculated Molecular Weight: 1257 aa, 140 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC126229
• Gene Symbol: L1CAM
• Gene ID (NCBI): 3897
• RRID: AB_2919423
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).

Publications

Species	Application	Title
human	IF	J Extracell Vesicles; Isolation and quantification of L1CAM-positive extracellular vesicles on a chip as a potential biomarker for Parkinson's Disease; Authors - Danyu Li