Kininogen-1 Recombinant antibody

Kininogen-1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241397C11

Cat no : 84175-5-RR

Synonyms

KNG1, HMWK, Fitzgerald factor, Bradykinin, BDK

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Validation Data Gallery

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  WB analysis of rat plasma using 84175-5-RR

  Rat plasma was subjected to SDS PAGE followed by western blot with 84175-5-RR (Kininogen-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 84175-5-RR

  Human plasma was subjected to SDS PAGE followed by western blot with 84175-5-RR (Kininogen-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HT-29 using 84175-5-RR

  1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Kininogen-1 Recombinant antibody (84175-5-RR, Clone:241397C11) (red), or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 84175-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84175-5-RR against Human Kininogen-1 were performed. The affinity constant is 0.182 nM.

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Tested Applications

• Positive WB detected in: rat plasma, human plasma

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84175-5-RR is Kininogen-1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: kininogen 1
• Calculated Molecular Weight: 72kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: NM_001102416.3
• Gene Symbol: Kininogen 1
• Gene ID (NCBI): 3827
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This KNG1 gene contains 10 exons and encodes two proteins through alternative splicing: high molecular weight kininogen (HMWK) (72kDa) and low molecular weight kininogen (LMWK) (48kDa) (PMID: 37442062). HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.