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Kininogen-1 Recombinant antibody, PBS Only

Kininogen-1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241397C11

Cat no : 84175-5-PBS

Synonyms

KNG1, HMWK, Fitzgerald factor, Bradykinin, BDK

Validation Data Gallery

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  • Western Blot (WB) analysis of rat plasma using Kininogen-1 Recombinant antibody (84175-5-RR)

    WB analysis of rat plasma using 84175-5-RR (same clone as 84175-5-PBS)

    Rat plasma was subjected to SDS PAGE followed by western blot with 84175-5-RR (Kininogen-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84175-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using Kininogen-1 Recombinant antibody (84175-5-RR)

    WB analysis of human plasma using 84175-5-RR (same clone as 84175-5-PBS)

    Human plasma was subjected to SDS PAGE followed by western blot with 84175-5-RR (Kininogen-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84175-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84175-5-RR

    Affinity and Kinetic Characterization of 84175-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84175-5-RR against Human Kininogen-1 were performed. The affinity constant is 0.182 nM.

Product Information

The immunogen of 84175-5-PBS is Kininogen-1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name kininogen 1
Calculated Molecular Weight72kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberNM_001102416.3
Gene Symbol Kininogen 1
Gene ID (NCBI) 3827
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This KNG1 gene contains 10 exons and encodes two proteins through alternative splicing: high molecular weight kininogen (HMWK) (72kDa) and low molecular weight kininogen (LMWK) (48kDa) (PMID: 37442062). HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.