Kindlin 1 Polyclonal antibody, PBS Only

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  WB analysis using 22215-1-AP (same clone as 22215-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22215-1-AP (Kindlin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 22215-1-AP (same clone as 22215-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22215-1-AP (FERMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 22215-1-AP (same clone as 22215-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22215-1-AP (FERMT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 22215-1-AP (same clone as 22215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (Kindlin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 22215-1-AP (same clone as 22215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (Kindlin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 22215-1-AP (same clone as 22215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (FERMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 22215-1-AP (same clone as 22215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 22215-1-AP (same clone as 22215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 22215-1-AP (same clone as 22215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 22215-1-AP (same clone as 22215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 22215-1-AP (same clone as 22215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (FERMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 22215-1-AP (same clone as 22215-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 22215-1-AP (FERMT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Kindlin 1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 22215-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

22215-1-PBS targets Kindlin 1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Kindlin 1 fusion protein Ag17543
• Full Name: fermitin family homolog 1 (Drosophila)
• Calculated Molecular Weight: 677 aa, 77 kDa
• Observed Molecular Weight: 70-77 kDa
• GenBank Accession Number: BC035882
• Gene Symbol: Kindlin 1
• Gene ID (NCBI): 55612
• RRID: AB_2879033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BQL6
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Kindlin-1 is a FERM domain containing adaptor protein that is found predominantly at cell-extracellular matrix adhesions where it binds to β-integrin subunits and is required for integrin activation. Loss of function mutations in the FERMT1 gene which encodes Kindlin-1 leads to the development of Kindler Syndrome (KS) an autosomal recessive skin disorder characterized by skin blistering, photosensitivity, and predisposition to aggressive squamous cell carcinoma (SCC).