CoraLite® Plus 647-conjugated KU70 Polyclonal antibody

• Featured Product
• KD/KO Validated

KU70 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-10723

Synonyms

XRCC6, CTC75, CTC box-binding factor 75 kDa subunit, ATP-dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase 2 subunit 1

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Validation Data Gallery

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  IF Staining of HeLa using CL647-10723

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 KU70 antibody (CL647-10723) at dilution of 1:200.

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  FC experiment of HepG2 using CL647-10723

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human KU70 (CL647-10723) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-10723 targets KU70 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KU70 fusion protein Ag1043
• Full Name: X-ray repair complementing defective repair in Chinese hamster cells 6
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC008343
• Gene Symbol: KU70
• Gene ID (NCBI): 2547
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.