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KU70,XRCC6 Polyclonal antibody
KU70,XRCC6 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse and More (1)
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 10723-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, MCF-7 cells, mouse colon tissue, A549 cells, Hela cells, mouse spleen tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human colon cancer tissue, mouse kidney tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 23 publications below |
IF | See 1 publications below |
IP | See 2 publications below |
CoIP | See 1 publications below |
Product Information
10723-1-AP targets KU70,XRCC6 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | KU70,XRCC6 fusion protein Ag1043 |
Full Name | X-ray repair complementing defective repair in Chinese hamster cells 6 |
Calculated molecular weight | 75 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC008343 |
Gene symbol | XRCC6 |
Gene ID (NCBI) | 2547 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.
Protocols
Product Specific Protocols | |
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WB protocol for KU70,XRCC6 antibody 10723-1-AP | Download protocol |
IHC protocol for KU70,XRCC6 antibody 10723-1-AP | Download protocol |
IF protocol for KU70,XRCC6 antibody 10723-1-AP | Download protocol |
IP protocol for KU70,XRCC6 antibody 10723-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oncogene UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy. | ||
Phytomedicine Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer | ||
J Mater Chem B Hollow PtCo alloy nanospheres as a high-Z and oxygen generating nanozyme for radiotherapy enhancement in non-small cell lung cancer | ||
FEBS J Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy | ||
FASEB J Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats. | ||
Front Oncol ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Jonathan (Verified Customer) (02-03-2019) | Worked well for Western Blotting with little background.
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