KU70,XRCC6 Polyclonal antibody

• Featured Product
• KD/KO Validated

KU70,XRCC6 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

24

Reviews (1)

Cat no : 10723-1-AP

Synonyms

5 dRP lyase Ku70, 70 kDa subunit of Ku antigen, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, KU70, ML8, Thyroid lupus autoantigen, TLAA, XRCC6

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Validation Data Gallery

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  WB analysis using 10723-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10723-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10723-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10723-1-AP (KU70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10723-1-AP

  IP Result of anti-KU70,XRCC6 (IP:10723-1-AP, 3ug; Detection:10723-1-AP 1:1000) with HeLa cells lysate 1600ug.

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  IHC staining of human colon cancer using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10723-1-AP (KU70 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 10723-1-AP (KU70 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of rat testis using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 10723-1-AP (KU70 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10723-1-AP (KU70 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10723-1-AP (KU70 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse kidney using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10723-1-AP (KU70 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10723-1-AP (KU70 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10723-1-AP (KU70 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10723-1-AP

  Immunofluorescent analysis of HepG2 cells, using XRCC6 antibody 10723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 10723-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KU70 antibody (10723-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 10723-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug KU70 antibody (10723-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: A431 cells, MCF-7 cells, mouse colon tissue, A549 cells, Hela cells, mouse spleen tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue, mouse kidney tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 23 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

10723-1-AP targets KU70,XRCC6 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KU70,XRCC6 fusion protein Ag1043
• Full Name: X-ray repair complementing defective repair in Chinese hamster cells 6
• Calculated molecular weight: 75 kDa
• Observed molecular weight: 70 kDa
• GenBank accession number: BC008343
• Gene symbol: XRCC6
• Gene ID (NCBI): 2547
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.

Publications

Species	Application	Title
human	WB,IF	Oncogene; UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy.; Authors - L Hu
human	WB	Phytomedicine; Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer; Authors - Meiyang Chen
human	WB	J Mater Chem B; Hollow PtCo alloy nanospheres as a high-Z and oxygen generating nanozyme for radiotherapy enhancement in non-small cell lung cancer; Authors - Shuying Li
human	WB	FEBS J; Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy; Authors - Yan Wei
rat	WB	FASEB J; Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats.; Authors - Catherine E Aiken
human	WB	Front Oncol; ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway; Authors - Xin Wen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jonathan (Verified Customer) (02-03-2019)

Worked well for Western Blotting with little background.

• Applications: Western Blot,