Cytokeratin 7 Polyclonal antibody

Cytokeratin 7 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 15539-1-AP

Synonyms

KRT7, keratin 7, Cytokeratin-7, Cytokeratin,Keratin, CK-7

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Validation Data Gallery

• 

  WB analysis using 15539-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15539-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15539-1-AP (KRT7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15539-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15539-1-AP (KRT7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 15539-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15539-1-AP (KRT7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15539-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15539-1-AP (KRT7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:25600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:25600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary tumor using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15539-1-AP (Cytokeratin 7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15539-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15539-1-AP (Cytokeratin 7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 15539-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Cytokeratin 7 antibody (15539-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15539-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 7 antibody (15539-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 15539-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Cytokeratin 7 Polyclonal antibody (15539-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, rat liver tissue, A431 cells, mouse liver tissue, HepG2 cells, MCF-7 cells
• Positive IHC detected in: human pancreas tissue, human stomach cancer tissue, human liver cancer tissue, mouse pancreas tissue, human breast cancer tissue, human ovary tumor tissue, human lung cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, human lung tissue, human bowen disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse pancreas tissue
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:30000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 10 publications below
• IF: See 12 publications below

Product Information

15539-1-AP targets Cytokeratin 7 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 7 fusion protein Ag7895
• Full Name: keratin 7
• Calculated Molecular Weight: 469 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC002700
• Gene Symbol: Cytokeratin 7
• Gene ID (NCBI): 3855
• RRID: AB_2249769
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytokeratin 7 (CK7) is a type II keratin protein that is a principal constituent of the intermediate filament cytoskeleton. It is primarily expressed in simple epithelia lining the cavities of internal organs, glandular ducts, and blood vessels. Abnormal expression of CK7 has been linked to various pathological conditions, including cancer. Overexpression of CK7 promotes tumor progression and metastasis in different human cancers, and its suppression leads to rapid tumor regression, highlighting its potential as a therapeutic target CK7 is also involved in inhibiting interferon-dependent interphase, promoting DNA synthesis, initiating translation possibly through interaction with p150 (the largest subunit of eukaryotic translation initiation factor 3), and interacting with G protein-coupled estrogen receptor 1 (GPER1), which activates several signaling pathways. In the context of cancer diagnosis, CK7 is used as a marker to distinguish between different types of carcinomas. It is expressed in most adenocarcinomas, particularly those of the lung and colorectal origin, and is useful in differentiating primary ovarian carcinoma from metastatic colorectal carcinoma. CK7 expression has also been studied as a predictor of an unfavorable prognosis in colorectal carcinoma.

Publications

Species	Application	Title
	IF	Cell; Neurulation of the cynomolgus monkey embryo achieved from 3D blastocyst culture; Authors - Jinglei Zhai
mouse	IHC	Cancer Cell; Promotion of cholangiocarcinoma growth by diverse cancer-associated fibroblast subpopulations.; Authors - Silvia Affo
human	IF	Cell Death Dis; α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation; Authors - Hao Wang
human	WB,IHC,IF	Apoptosis; PANoptosis subtypes predict prognosis and immune efficacy in gastric cancer; Authors - Zitao Liu
human	WB, IHC, IF	Cell Death Dis; LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface.; Authors - S Liu
mouse	IHC	iScience; Cooperation between PRMT1 and PRMT6 drives lung cancer health disparities among Black/African American men; Authors - Pei-Ying Wu

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