Cytokeratin 18 Polyclonal antibody, PBS Only

Cytokeratin 18 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10830-1-PBS

Synonyms

KRT18, keratin 18, Cytokeratin,Keratin, CK-18, CK18

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Validation Data Gallery

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  WB analysis using 10830-1-AP (same clone as 10830-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 10830-1-AP (same clone as 10830-1-PBS)

  IP result of anti-Cytokeratin 18 (IP:10830-1-AP, 3ug; Detection:10830-1-AP 1:2000) with A431 cells lysate 3300ug. This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate hyperplasia using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate hyperplasia using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10830-1-AP (same clone as 10830-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 10830-1-AP (same clone as 10830-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cytokeratin 18 antibody (10830-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 10830-1-AP (same clone as 10830-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 18 antibody (10830-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 10830-1-AP (same clone as 10830-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Cytokeratin 18 Polyclonal antibody (10830-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10830-1-PBS in a different storage buffer formulation.

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Product Information

10830-1-PBS targets Cytokeratin 18 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 18 fusion protein Ag1260
• Full Name: keratin 18
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC000180
• Gene Symbol: Cytokeratin 18
• Gene ID (NCBI): 3875
• RRID: AB_2133164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.