Cytokeratin 17-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

Cytokeratin 17-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 17516-1-AP

Synonyms

39.1, Cell and organelle markers, CK 17, Cytokeratin 17, Cytokeratin 17-Specific, Cytoskeleton Marker, K17, keratin 17, KRT17, PCHC1

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Validation Data Gallery

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  WB analysis using 17516-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 17516-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 17516-1-AP (Cytokeratin 17-Specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17516-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17516-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 17516-1-AP

  IP Result of anti-Cytokeratin 17-Specific (IP:17516-1-AP, 4ug; Detection:17516-1-AP 1:1000) with A431 cells lysate 1200ug.

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  IHC staining of human cervical cancer using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cervical cancer using 17516-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 17516-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 17-Specific antibody (17516-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 17516-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 17-Specific antibody (17516-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 17516-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Cytokeratin 17-Specific antibody (17516-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: A431 cells, mouse skin tissue, HeLa cells, HEK-293 cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human cervical cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:300-1:1200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 9 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

17516-1-AP targets Cytokeratin 17-Specific in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 17
• Calculated molecular weight: 48 kDa
• Observed molecular weight: 48 kDa
• GenBank accession number: NM_000422
• Gene symbol: KRT17
• Gene ID (NCBI): 3872
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.

Publications

Species	Application	Title
mouse		Science; Spatiotemporal immune zonation of the human kidney.; Authors - Benjamin J Stewart
human	IHC	Clin Cancer Res; A Preclinical Trial and Molecularly Annotated Patient Cohort Identify Predictive Biomarkers in Homologous Recombination-deficient Pancreatic Cancer.; Authors - Yifan Wang
human	WB	Breast Cancer Res; Anillin regulates breast cancer cell migration, growth, and metastasis by non-canonical mechanisms involving control of cell stemness and differentiation.; Authors - Dongdong Wang
human	WB	Oncogene; The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer; Authors - Mengjie Li ; KD Validated
human,mouse	WB	J Invest Dermatol; Keratin 17 Impacts Global Gene Expression and Controls G2/M Cell Cycle Transition in Ionizing Radiation-Induced Skin Damage; Authors - ZhiCao Yue
human	CoIP	Int J Mol Sci; APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.; Authors - Marianna Naki