CoraLite®555-conjugated Cytokeratin 17-Specific Polyclonal antibody

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• KD/KO Validated

Cytokeratin 17-Specific Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Reviews (1)

Cat no : CL555-17516

Synonyms

39.1, Cell and organelle markers, CK 17, Cytokeratin 17, Cytokeratin 17-Specific, Cytoskeleton Marker, K17, keratin 17, KRT17, PC, PC2, PCHC1

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Validation Data Gallery

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  IF Staining of HeLa using CL555-17516

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 Cytokeratin 17-Specific antibody (CL555-17516) at dilution of 1:200.

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  FC experiment of HeLa using CL555-17516

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Cytokeratin 17-Specific (CL555-17516) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-17516 targets Cytokeratin 17-Specific in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 17
• Calculated Molecular Weight: 48 kDa
• GenBank Accession Number: NM_000422
• Gene Symbol: Cytokeratin 17
• Gene ID (NCBI): 3872
• RRID: AB_2919634
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Davide (Verified Customer) (07-26-2024)

The anibody was very specific and results suitable for multiplexed imaging. Worked well on wholemount peeled oesophageal epithelium.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Oesophagus

CL555 Cytokeratin 17-Specific Antibody Immunofluorescence validation (1:200 dilution) in Oesophagus (Cat no:CL555-17516)

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C