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  • KD/KO Validated

Cytokeratin 17-Specific Polyclonal antibody

Cytokeratin 17-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17516-1-AP

Synonyms

39.1, Cell and organelle markers, CK 17, Cytokeratin 17, Cytokeratin 17-Specific, Cytoskeleton Marker, K17, keratin 17, KRT17, PCHC1



Tested Applications

Positive WB detected inA431 cells, mouse skin tissue, HeLa cells, HEK-293 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman cervical cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:300-1:1200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

17516-1-AP targets Cytokeratin 17-Specific in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name keratin 17
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberNM_000422
Gene symbol KRT17
Gene ID (NCBI) 3872
RRIDAB_2133036
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 17-Specific antibody 17516-1-APDownload protocol
IHC protocol for Cytokeratin 17-Specific antibody 17516-1-APDownload protocol
IF protocol for Cytokeratin 17-Specific antibody 17516-1-APDownload protocol
IP protocol for Cytokeratin 17-Specific antibody 17516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Science

Spatiotemporal immune zonation of the human kidney.

Authors - Benjamin J Stewart
humanIHC

Clin Cancer Res

A Preclinical Trial and Molecularly Annotated Patient Cohort Identify Predictive Biomarkers in Homologous Recombination-deficient Pancreatic Cancer.

Authors - Yifan Wang
humanWB

Breast Cancer Res

Anillin regulates breast cancer cell migration, growth, and metastasis by non-canonical mechanisms involving control of cell stemness and differentiation.

Authors - Dongdong Wang
humanWB

Oncogene

The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer

Authors - Mengjie Li
  • KD Validated
mouseWB

Phytother Res

Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization

Authors - Yongling Ning
human,mouseWB

J Invest Dermatol

Keratin 17 Impacts Global Gene Expression and Controls G2/M Cell Cycle Transition in Ionizing Radiation-Induced Skin Damage

Authors - ZhiCao Yue