Cytokeratin 13 Recombinant antibody, PBS Only (Capture)

Cytokeratin 13 Recombinant Antibody for WB, IF, IHC, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

230366B10

Cat no : 83058-1-PBS

Synonyms

CK 13, CK13, Cytokeratin 13, K13, keratin 13, KRT13

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Validation Data Gallery

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  WB analysis using 83058-1-RR (same clone as 83058-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83058-1-RR (KRT13 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skin using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skin using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 83058-1-RR (same clone as 83058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  IF Staining of HaCaT using 83058-1-RR (same clone as 83058-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 13 antibody (83058-1-RR, Clone: 230366B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83058-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00074-1

  Cytometric bead array standard curve ofMP00074-1, KRT13 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83058-1-PBS. Detection antibody: 83058-3-PBS. Standard: Ag0217. Range: 0.625-20 ng/mL.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83058-1-PBS targets Cytokeratin 13 as part of a matched antibody pair:

MP00074-1: 83058-1-PBS capture and 83058-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cytokeratin 13 fusion protein Ag0217
• Full Name: keratin 13
• Calculated Molecular Weight: 50 kDa
• GenBank Accession Number: BC002661
• Gene Symbol: KRT13
• Gene ID (NCBI): 3860
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.