Cytokeratin 13 Recombinant antibody

• Featured Product
• KD/KO Validated

Cytokeratin 13 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230366B10

Cat no : 83058-1-RR

Synonyms

KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13

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Validation Data Gallery

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  WB analysis using 83058-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83058-1-RR (KRT13 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 83058-1-RR

  WB result of Cytokeratin 13 antibody (83058-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 13 transfected A431 cells.

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  IHC staining of human urothelial carcinoma using 83058-1-RR

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 83058-1-RR

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 83058-1-RR

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 83058-1-RR

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 83058-1-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 83058-1-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 83058-1-RR

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 83058-1-RR

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 83058-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 83058-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83058-1-RR (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HaCaT using 83058-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 13 antibody (83058-1-RR, Clone: 230366B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 83058-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83058-1-RR against Human Cytokeratin 13 were performed. The affinity constant is 1.99 nM.

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Tested Applications

• Positive WB detected in: HaCaT cells, A431 cells, mouse skin tissue, rat skin tissue, rat thymus tissue
• Positive IHC detected in: human urothelial carcinoma tissue, rat skin tissue, mouse skin tissue, human oesophagus cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HaCaT cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83058-1-RR targets Cytokeratin 13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cytokeratin 13 fusion protein Ag0217
• Full Name: keratin 13
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC002661
• Gene Symbol: Cytokeratin 13
• Gene ID (NCBI): 3860
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.