WB result of KPNA4 antibody (82898-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KPNA4 transfected A549 cells.
WB analysis using 82898-2-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82898-2-RR (KPNA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 82898-2-RR (KPNA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human intrahepatic cholangiocarcinoma using 82898-2-RR
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82898-2-RR (KPNA4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82898-2-RR (KPNA4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS using 82898-2-RR
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KPNA4 antibody (82898-2-RR, Clone: 230098H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KPNA4 antibody (82898-2-RR, Clone: 230098H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of A549 using 82898-2-RR
1x10^6 A549 cells were intracellularly stained with 0.25 ug KPNA4 Recombinant antibody (82898-2-RR, Clone:230098H1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1x10^6 A549 cells were intracellularly stained with 0.25 ug KPNA4 Recombinant antibody (82898-2-RR, Clone:230098H1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
Affinity and Kinetic Characterization of 82898-2-RR
Biolayer interferometry (BLl) kinetic assays of 82898-2-RR against Human KPNA4 were performed. The affinity constant is 0.678 nM.
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HeLa cells, A549 cells, Jurkat cells, NIH/3T3 cells, mouse ovary tissue, mouse testis tissue, rat testis tissue
Positive IHC detected in
human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
U2OS cells
Positive FC (Intra) detected in
A549 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
82898-2-RR targets KPNA4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
KPNA4, also named importin subunit alpha-3, karyopherin-alpha4, is a cytoplasmic protein that recognizes nuclear localization signals (NLSs) and dock NLS-containing proteins to the nuclear pore complex. Nuclear import, mediated in part by karyopherin-α (KPNA)/importin-α subtypes, regulates transcription factor access to the genome and determines cell fate. KPNA4-mediated nuclear transport of Ras-responsive element-binding protein (RREB1), which sustains Ras/ERK pathway signaling through repressing miR-143/145 expression (PMID: 31822798). KPNA4 is one of the main isoforms that is activated in many human cancers. KPNA4 expression was elevated in head and neck of squamous cell carcinoma (PMID: 33188837).
WB result of KPNA4 antibody (82898-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KPNA4 transfected A549 cells.
WB analysis using 82898-2-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82898-2-RR (KPNA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human intrahepatic cholangiocarcinoma using 82898-2-RR
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82898-2-RR (KPNA4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of U2OS using 82898-2-RR
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KPNA4 antibody (82898-2-RR, Clone: 230098H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC (INTRA) Figures
FC experiment of A549 using 82898-2-RR
1x10^6 A549 cells were intracellularly stained with 0.25 ug KPNA4 Recombinant antibody (82898-2-RR, Clone:230098H1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
AFFINITY Figures
Affinity and Kinetic Characterization of 82898-2-RR
Biolayer interferometry (BLl) kinetic assays of 82898-2-RR against Human KPNA4 were performed. The affinity constant is 0.678 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
KPNA4 Recombinant antibody
Catalog Number
82898-2-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 IHC : 1:50-1:500 IF/ICC : 1:50-1:500 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
