KNOP1 Polyclonal antibody

KNOP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30115-1-AP

Synonyms

101F10.1, C16orf88, FAM178A, Protein C16orf88, TSG118

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Validation Data Gallery

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  WB analysis using 30115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30115-1-AP (KNOP1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30115-1-AP (KNOP1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 30115-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KNOP1 antibody (30115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: K-562 cells, U-251 cells, MCF-7 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30115-1-AP targets KNOP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KNOP1 fusion protein Ag32395
• Full Name: chromosome 16 open reading frame 88
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_001012991.2
• Gene Symbol: KNOP1
• Gene ID (NCBI): 400506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lysine-rich nucleolar protein 1 (KNOP1) is a nucleolar protein that interacts with zinc finger 106 protein. Also known as Tsg118, it was first reported in 1999 (3). KNOP1 is predominantly expressed in proliferating somatic cells and in male germ cells, and may therefore be involved in testicular development. (PMID: 37588747)